Application of non-traditional yeasts in the improvement of bioethanol production from inuline containing raw materials - biotechnological and systems biology approach
Project No. 2DP/18.104.22.168.0/14/APIA/VIAA/043, LU Nr. ESS2014/171
Source of funding: ERAF
Project period: 01.08.2014. – 31.08.2015.
Biosystems group is joining the project of Institute of Microbiology and Biotechnology of University of Latvia under the leadership of Dr.biol. Armands Vigants. We contribute by development of central metabolism model of Kluyveromyces marxianus and modelling of possible metabolic engineering activities.
Application of systems biology approach is applied to improve the biotechnological process to improve inulin containing substrate conversion into ethanol using unconventional yeast Kluyveromyces marxianus.
Laboratory of Bioconversion of Carbohydrates, Institute of Microbiology and Biotechnology at University of Latvia (Armands Vigants)
Laboratory of Yeast Nutritional Physiology, Institute of Microbiology and Biotechnology, University of Latvia (Janis Liepins)
The biotechnological potential of K. marxianus is assessed by a constraint based stoichiometric modelling approach. A biomass-coupled model of central metabolism was developed to be a basis for the design of metabolic engineering and to assess in silico the production of ethanol, acetate, lactate, glycerol, ethylacetate, succinate, glutamate phenylethanol, phenylalanine.